Greater Boston Trout Unlimited (GBTU) is partnering with the Neponset River Watershed Association (NepWRA) to map and track the health of the Neponset River’s cold water tributaries and the wild brook and other trout that call them home. The project will assess habitat quality, inventory barriers to passage, and evaluate the trout populations of the watershed’s cold-water tributaries using cutting-edge environmental DNA (eDNA) technology. Based on our findings, the Massachusetts Division of Fisheries and Wildlife will conduct a full-scale fish population assessment and partner in long-term strategic conservation plans for the watershed. GBTU member-volunteers, Boston urban youth, and the 14 communities through which the Neponset flows will be involved in every phase of the project. The project will be supervised by GBTU Board members Gabby Queenan, Policy Director of the Massachusetts Rivers Association (MRA); and Bill Pastuszek, immediate GBTU past president, Conservation Committee co-chair, and co-chair NLC Land Conservancy workgroup. Chris Hirsch, GBTU Board member and the NepRWA Environmental Scientist, will serve as the scientific coordinator and liaison among the partner organizations.